Composite

Part:BBa_K2715007:Design

Designed by: Daniel Partridge   Group: iGEM18_Nottingham   (2018-10-08)


Antisense RNA targeting C. difficile toxins composite 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Synthetic antisense RNA parts BBa_K2715017 and BBa_K2715015 are transcribed from strong promoters BBa_K2715010 and BBa_K2715011 respectively. Both antisense RNA parts are synthetic and were designed to target two important C. difficile toxins TcdA (part BBa_K2715015) and TcdB (part BBa_K2715017).


Source

The two clostridial promoters were taken from C. acetobutylicum and C. sporogenes, and the antisense RNA fragments were synthesised by IDT.